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Issue 15, 2012
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Generating contrast in hyperpolarized 13C MRI using ligand–receptor interactions

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Abstract

We report the imaging of β-cyclodextrin–benzoic acid binding at 14T using hyperpolarized 13C magnetic resonance (MR). Benzoic acid was polarized using a dynamic nuclear polarization (DNP) approach and combined with β-cyclodextrin in aqueous solution. As anticipated, decreases in the spin–lattice relaxation constant (T1) were observed with decreases in the ligand–receptor ratio. The calculated log K was approximately 1.7, similar to previously reported binding constants. Hyperpolarized [1-13C] benzoic acid was used to interrogate solutions of variable β-cyclodextrin concentrations, with the mixtures imaged at 14T using a 3D frequency-selective MR sequence. Differences in β-cyclodextrin concentration were easily visualized. These results suggest that hyperpolarized 13C MR could be used in vivo to determine the presence and density of receptors for a given ligand–receptor pair.

Graphical abstract: Generating contrast in hyperpolarized 13C MRI using ligand–receptor interactions

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Publication details

The article was received on 27 Mar 2012, accepted on 03 May 2012 and first published on 04 May 2012


Article type: Communication
DOI: 10.1039/C2AN35406C
Citation: Analyst, 2012,137, 3427-3429
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    Generating contrast in hyperpolarized 13C MRI using ligand–receptor interactions

    K. R. Keshari, J. Kurhanewicz, J. M. Macdonald and D. M. Wilson, Analyst, 2012, 137, 3427
    DOI: 10.1039/C2AN35406C

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