Institute of Molecular Science, Chemical Biology and Molecular Engineering, Laboratory of Education Ministry, Shanxi University, Taiyuan 030006, P.R. China
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Analyst, 2012,137, 3160-3165
13 Jan 2012,
18 Apr 2012
First published online
20 Apr 2012
This work reports a sensitive amperometric biosensor for organophosphate pesticides (OPs) fabricated by modifying a glassy carbon electrode with acetylcholinesterase (AChE) immobilized on ionic liquid-functionalized graphene (IL-G). The functionalized graphene sheets had good dispersibility and long-term stability in various solvents. The as-prepared biosensor showed high affinity to acetylthiocholine (ATCl) with a Michaelis–Menten constant (Km) value of 0.77 mM. Furthermore, based on the inhibition by OPs of the enzymatic activity of the immobilized AChE, and using carbaryl as a model compound, the inhibition of carbaryl was proportional to its concentration ranging from 0.0025 to 0.48 and 0.48 to 1.42 μg mL−1 with a detection limit of 0.8 ng mL−1 (S/N = 3). The developed biosensor exhibited a good performance for OPs detection, including good reproducibility and acceptable stability, which provided a new and promising tool for the analysis of enzyme inhibitors.
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