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Issue 13, 2012
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Expression of membrane-associated proteins within single emulsion cell facsimiles

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Abstract

MreB is a structural membrane-associated protein which is one of the key components of the bacterial cytoskeleton. Although it plays an important role in shape maintenance of rod-like bacteria, the understanding of its mechanism of action is still not fully understood. This study shows how segmented flow and microdroplet technology can be used as a new tool for biological in vitro investigation of this protein. In this paper, we demonstrate cell-free expression in a single emulsion system to express red fluorescence protein (RFP) and MreB linked RFP (MreB–RFP). We follow the aggregation and localisation of the fusion protein MreB–RFP in this artificial cell-like environment. The expression of MreB–RFP in single emulsion droplets leads to the formation of micrometer-scale protein patches distributed at the water/oil interface.

Graphical abstract: Expression of membrane-associated proteins within single emulsion cell facsimiles

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Publication details

The article was received on 10 Jan 2012, accepted on 02 Mar 2012, published on 05 Mar 2012 and first published online on 05 Mar 2012


Article type: Paper
DOI: 10.1039/C2AN35047E
Citation: Analyst, 2012,137, 2939-2943
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    Expression of membrane-associated proteins within single emulsion cell facsimiles

    M. Chanasakulniyom, C. Martino, D. Paterson, L. Horsfall, S. Rosser and J. M. Cooper, Analyst, 2012, 137, 2939
    DOI: 10.1039/C2AN35047E

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