We demonstrate a new dual slope SPR technique that is ten-fold faster than the conventional step-response method. The new scheme utilizes rapid slope-based measurements followed by rapid reset, and it separates association and dissociation half reaction measurements at two separate sites inside a dual-chamber PDMS microfluidic chip. For a model CAII-ABS test system, the association and dissociation slopes were measured in 30 seconds compared to 5 minutes for step-response. The values of ka and kd calculated from the slope method are 3.66 ± 0.19 × 103 M−1 s−1 and 4.83 ± 0.17 × 10−2 s−1, respectively, matching well with step-response values while facilitating 10 to 15 fold faster detection and quantification.
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