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Issue 11, 2012
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Novel colorimetric assay of LSD1 activity using gold nanoparticles

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Abstract

We have developed a simple and sensitive strategy for colorimetric LSD1 enzyme activity assay using avidin modified gold nanoparticles. The strategy is based on the vivid color change of a gold nanoparticle solution from red to violet upon addition of a test solution of peptide–antibody treated with LSD1. Thus, the presence of LSD1 in a sample can be determined by simple visual inspection with the naked eye. In addition, a wide range of LSD1 concentrations (13 pM to 0.13 μM) were quantitatively determined by spectrophotometry, which shows the possibility of quantitative analysis of the over expression levels of LSD1 in cancer tissue samples.

Graphical abstract: Novel colorimetric assay of LSD1 activity using gold nanoparticles

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Publication details

The article was received on 22 Dec 2011, accepted on 29 Mar 2012 and first published on 02 Apr 2012


Article type: Paper
DOI: 10.1039/C2AN16286E
Citation: Analyst, 2012,137, 2669-2673
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    Novel colorimetric assay of LSD1 activity using gold nanoparticles

    J. Y. Piao and D. S. Chung, Analyst, 2012, 137, 2669
    DOI: 10.1039/C2AN16286E

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