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Highly ordered macroporous electrodes are prepared by electro-deposition of gold through a polystyrene sphere template. Drop-coatingredoxpolymer and either glucose oxidase, for the anode, or Melanocarpus albomyceslaccase, for the cathode on the macroporous gold provides film-coated electrodes for assembly of membrane-less glucose/oxygen enzymatic fuelcells (EFC) in pH 7.4 buffer containing 10 mM glucose and 0.15 M NaCl. Under these conditions the maximum power density of 17 μWcm−2 for EFCs using films adsorbed to planar goldelectrodes increased to 38 μWcm−2 for films adsorbed to 2½ sphere gold macroporous electrodes.
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