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Issue 7, 2011
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Human macrophage adhesion on polysaccharide patterned surfaces

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Abstract

Despite many advances in designing biocompatible materials, inflammation remains a problem in medical devices and implants. We report two methods, microcontact printing and photodegradation by UV exposure, to pattern dextran and hyaluronic acid on glass, as well as demonstrate their utility for use as an anti-inflammatory biomaterial. The dextran/glass patterned surface can be further modified by grafting hyaluronic acid to glass, creating a binary polysaccharide patterned surface. We used two geometries, 90 μm squares and 22 μm stripes, to study the human macrophage (THP-1) adhesion on the patterned surfaces containing dextran, hyaluronic acid and the binary pattern. The results indicate that a majority of the macrophages are non-adherent on hyaluronic acid for three day culture. The ranking of surfaces according to macrophage adhesion is 3-aminopropyltriethoxysilane-modified glass culture dish, dextranized surfaces, glass, and hyaluronic acid-modified surfaces. On the binary pattern of dextran and hyaluronic acid, macrophages preferentially attach and adhere to the dextranized area. Patterned surfaces provide an excellent platform for mimicking the complexity of the glycocalyx and investigating the interface between this surface and cells. This binary polysaccharide pattern also offers a new route to address anti-inflammatory potential of surface coatings on biomaterials in a high through-put fashion.

Graphical abstract: Human macrophage adhesion on polysaccharide patterned surfaces

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Publication details

The article was received on 21 Nov 2010, accepted on 01 Feb 2011 and first published on 23 Feb 2011


Article type: Paper
DOI: 10.1039/C0SM01353F
Citation: Soft Matter, 2011,7, 3599-3606
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    Human macrophage adhesion on polysaccharide patterned surfaces

    I. Y. Tsai, C. Kuo, N. Tomczyk, S. J. Stachelek, R. J. Composto and D. M. Eckmann, Soft Matter, 2011, 7, 3599
    DOI: 10.1039/C0SM01353F

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