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Issue 2, 2011
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Vacuum soft lithography to direct neuronal polarization

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The ability to coat surfaces with pre-determined patterns of biomolecules by soft lithography has found use in areas ranging from fundamental biology to translational medicine, such as tissue engineering and diagnostics. However, existing surface patterning techniques (e.g., microcontact printing and traditional lithography) are unable to pattern several biomolecules in a single step. Here we introduce a simple method to simultaneously pattern multiple biomolecules in complex two-dimensional configurations onto substrates with better than 2 µm resolution. This protocol, termed vacuum soft lithography, utilized below ambient pressures temporarily stored within a removable microfluidic template to expose specific regions of a substrate to multiple biochemical solutions. We demonstrate the utility of this vacuum soft lithography technique by fabricating a multi-component array that directs the adhesion, polarization, and neurite guidance of primary hippocampal neurons.

Graphical abstract: Vacuum soft lithography to direct neuronal polarization

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The article was received on 27 Aug 2010, accepted on 04 Oct 2010 and first published on 25 Oct 2010

Article type: Communication
DOI: 10.1039/C0SM00869A
Citation: Soft Matter, 2011,7, 343-347
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    Vacuum soft lithography to direct neuronal polarization

    J. T. Nevill, A. Mo, B. J. Cord, T. D. Palmer, M. Poo, L. P. Lee and S. C. Heilshorn, Soft Matter, 2011, 7, 343
    DOI: 10.1039/C0SM00869A

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