Jump to main content
Jump to site search

Issue 14, 2011
Previous Article Next Article

Molecular mechanisms of phosphatidylcholine monolayer solidification due to hydroxyl radicals

Author affiliations

Abstract

The reaction of L-α-1,2-dipalmitoylphosphatidylcholine (DPPC) with hydroxyl radicals (HO˙ from Fenton solutions) was investigated using monolayer techniques: isotherms, infrared reflection absorption spectroscopy (IRRAS), grazing incidence X-ray diffraction, X-ray reflection and fluorescence microscopy. The DPPC monolayer is attacked with different HO˙ concentrations. The decrease in the lateral pressure was used as a measure of the efficiency of the HO˙ attack. With increasing HO˙ concentration, the plateau region in the isotherm was shifted to a lower surface pressure; eventually it disappeared. Fluorescence microscopy during the HO˙ attack showed that new domains in the condensed phase nucleate immediately. With isotherms and X-ray diffraction we found that the monolayer can be compressed to smaller molecular areas, with a reduced tilt angle of the alkyl chains. IRRAS experiments indicated a partial cleavage of the head group leading to a reduced head group size.

Graphical abstract: Molecular mechanisms of phosphatidylcholine monolayer solidification due to hydroxyl radicals

Back to tab navigation

Publication details

The article was received on 21 Feb 2011, accepted on 06 May 2011 and first published on 08 Jun 2011


Article type: Paper
DOI: 10.1039/C1SM05312D
Citation: Soft Matter, 2011,7, 6467-6476
  •   Request permissions

    Molecular mechanisms of phosphatidylcholine monolayer solidification due to hydroxyl radicals

    A. Gröning, H. Ahrens, T. Ortmann, F. Lawrenz, G. Brezesinski, F. Scholz and C. A. Helm, Soft Matter, 2011, 7, 6467
    DOI: 10.1039/C1SM05312D

Search articles by author

Spotlight

Advertisements