Jump to main content
Jump to site search

Issue 5, 2011
Previous Article Next Article

Toehold of dsDNA exchange affects the hydrogel swelling kinetics of a polymer–dsDNA hybrid hydrogel

Author affiliations

Abstract

Hydrogel-based label-free nucleic acids sensor materials using high resolution interferometric readout of hydrogel swelling changes was prepared and characterized with respect to molecular design parameters. The DNA-sensitive hydrogel comprised sensing (S) and blocking (B) oligonucleotide pairs copolymerized with the network and formed reversible crosslinks in addition to stable covalent ones. Oligonucleotide probes (P) complementary to S with longer complementary regions comparing to B results in competitive replacement of S–B strands. The associated destabilization of DNA crosslinks results in changes of the hydrogel swelling. S–B dioligonucleotidic crosslinkers were designed with 8, 12 and 16 basepairs in complementary regions and were destabilized by probes with matching sequences with 2, 6 and 10 excess basepairs in the so-called “toe-hold” region. The kinetics of hydrogel swelling was tested at room and body temperature for hydrogels with different covalent crosslink densities. In general, the swelling kinetics was faster for longer “toe-holds” and slower for the longer and more stable blocking sequence. An increase of the covalent crosslink density resulted in a decrease of the swelling rate. The swelling of the hydrogel was faster the higher the temperature and closer to the S–B melting point. The following work shows direct correlation between the kinetics of strand displacement reaction and hydrogel swelling rates and provides a direction for further application of DNA-sensitive hydrogels.

Graphical abstract: Toehold of dsDNA exchange affects the hydrogel swelling kinetics of a polymer–dsDNA hybrid hydrogel

Back to tab navigation

Publication details

The article was received on 02 Sep 2010, accepted on 03 Nov 2010 and first published on 20 Dec 2010


Article type: Paper
DOI: 10.1039/C0SM00915F
Citation: Soft Matter, 2011,7, 1741-1746
  •   Request permissions

    Toehold of dsDNA exchange affects the hydrogel swelling kinetics of a polymer–dsDNA hybrid hydrogel

    M. Gao, K. Gawel and B. T. Stokke, Soft Matter, 2011, 7, 1741
    DOI: 10.1039/C0SM00915F

Search articles by author

Spotlight

Advertisements