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Issue 6, 2011
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All-optical fluorescence image recovery using modulated stimulated emission depletion

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Abstract

Fluorescence modulation for selective recovery of desired fluorescence signals has to date required careful fluorophore selection combined with repeated optical recovery from long-lived photoinduced dark states. Adapting an all-optical scheme, modulated stimulated emission depletion (STED) generalizes such modulation schemes by eliminating the need for dark state residence by directly optically depopulating the emissive state at any externally applied frequency. Using two overlapped Gaussian laser spots with the depletion beam being intensity-modulated, fluorescence modulation is readily achieved with a depletion ratio governed by the intensity of the depleting laser. Selective image recovery of otherwise unmodulatable fluorophore signals is directly achieved through this all-optical modulation, and common STED-degrading multiphoton-excited background is readily discriminated against. Both beads and dyes in solution, as well as fluorophores bound within fixed cells are readily imaged in this manner.

Graphical abstract: All-optical fluorescence image recovery using modulated stimulated emission depletion

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Publication details

The article was received on 20 Dec 2010, accepted on 14 Mar 2011 and first published on 28 Mar 2011


Article type: Edge Article
DOI: 10.1039/C0SC00637H
Citation: Chem. Sci., 2011,2, 1080-1085
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    All-optical fluorescence image recovery using modulated stimulated emission depletion

    C. Fan, J. Hsiang, A. E. Jablonski and R. M. Dickson, Chem. Sci., 2011, 2, 1080
    DOI: 10.1039/C0SC00637H

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