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Issue 3, 2011
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Genetically encoding an aliphatic diazirine for protein photocrosslinking

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Abstract

Photocrosslinking is an important approach that allows discovery and detailed investigation of proteinprotein, proteinoligonucleotide, and protein–small molecule interactions with high temporal and spatial resolution. A major limitation to the universal application of this methodology is the site-specific introduction of efficient aliphatic photocrosslinking probes into proteins of interest. Here, we report a novel aliphatic diazirine amino acid and its genetically encoded, site-specific incorporation into proteins in bacterial and mammalian cells. Furthermore, we demonstrate efficient photocrosslinking of a test proteinin vitro and in vivo.

Graphical abstract: Genetically encoding an aliphatic diazirine for protein photocrosslinking

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Publication details

The article was received on 15 Jul 2010, accepted on 15 Nov 2010 and first published on 01 Dec 2010


Article type: Edge Article
DOI: 10.1039/C0SC00373E
Citation: Chem. Sci., 2011,2, 480-483
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    Genetically encoding an aliphatic diazirine for protein photocrosslinking

    C. Chou, R. Uprety, L. Davis, J. W. Chin and A. Deiters, Chem. Sci., 2011, 2, 480
    DOI: 10.1039/C0SC00373E

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