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Issue 11, 2011
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Photodynamic inactivation of Penicillium chrysogenum conidia by cationic porphyrins

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Abstract

This work reports the photophysical and biological evaluation of five cationic porphyrins as photosensitizers (PS) for the photodynamic inactivation (PDI) of Penicillium chrysogenum conidia. Two different cationic porphyrin groups were synthesized from 5,10,15,20-tetrakis(4-pyridyl)porphyrin and 5,10,15,20-tetrakis(pentafluorophenyl)porphyrin. The photostability and singlet oxygen generation studies showed that these molecules are photostable and efficient singlet oxygen generators. PDI experiments of P. chrysogenum conidia conducted with 50 μmol L−1 of photosensitiser under white light at a fluence rate of 200 mW cm−2 over 20 min showed that the most effective PS caused a 4.1 log reduction in the concentration of viable conidia. The present results show that porphyrins 1a and 1b are more efficient PSs than porphyrin 2a while porphyrins 1c and 2b show no inactivation of P. chrysogenum. It is also clear that the effectiveness of the molecule as PS for antifungal PDI is strongly related with the porphyrin substituent groups, and consequently their solubility in physiological media. The average amount of PS adsorbed per viable conidium was a determining factor in the photoinactivation efficiency and varied between the different studied PSs. Cationic PSs 1a and 1b might be promising anti-fungal PDI agents with potential applications in phytosanitation, biofilm control, bioremediation, and wastewater treatment.

Graphical abstract: Photodynamic inactivation of Penicillium chrysogenum conidia by cationic porphyrins

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Publication details

The article was received on 08 Jun 2011, accepted on 22 Jun 2011 and first published on 22 Aug 2011


Article type: Paper
DOI: 10.1039/C1PP05174A
Citation: Photochem. Photobiol. Sci., 2011,10, 1735-1743
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    Photodynamic inactivation of Penicillium chrysogenum conidia by cationic porphyrins

    M. C. Gomes, S. M. Woranovicz-Barreira, M. A. F. Faustino, R. Fernandes, M. G. P. M. S. Neves, A. C. Tomé, N. C. M. Gomes, A. Almeida, J. A. S. Cavaleiro, Â. Cunha and J. P. C. Tomé, Photochem. Photobiol. Sci., 2011, 10, 1735
    DOI: 10.1039/C1PP05174A

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