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Issue 19, 2011
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Cationic polyfluorenes for intracellular delivery of proteins

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Two cationic polyfluorene derivatives, quaternary amine 1 and guanidine 2 sheathed systems, were prepared as potential carriers to mediate import of proteins into cells without requiring covalent attachment to the protein. Neither polymer showed significant cytotoxicities (IC50 100 μM) when exposed to Clone 9 rat liver cells. Both polymers were shown to mediate import of a series of four proteins chosen because they have different pI values, sizes, and variable organic fluor attachments. Once inside the cells, the quaternary amine system 1 released more of its cargo into regions outside the lysosomes. In one exploratory experiment, pyrenebutyrate was shown to accelerate import of a protein system by polymer 1.

Graphical abstract: Cationic polyfluorenes for intracellular delivery of proteins

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Publication details

The article was received on 01 Jun 2011, accepted on 08 Jul 2011 and first published on 16 Aug 2011

Article type: Communication
DOI: 10.1039/C1OB05874F
Citation: Org. Biomol. Chem., 2011,9, 6513-6518
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    Cationic polyfluorenes for intracellular delivery of proteins

    A. Kamkaew, R. Barhoumi, R. C. Burghardt and K. Burgess, Org. Biomol. Chem., 2011, 9, 6513
    DOI: 10.1039/C1OB05874F

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