Jump to main content
Jump to site search

Issue 17, 2011
Previous Article Next Article

Irreversible inhibitors and activity-based probes as research tools in chemical glycobiology

Author affiliations

Abstract

In this review, we will discuss the enzymes that are involved in the synthesis and degradation of glycoconjugates and we will give an overview of the inhibitors and activity-based probes (ABPs) that have been used to study these. Following discussion of some general aspects of the biosynthesis and degradation of N-linked glycoproteins, attention is focused on the enzymes that hydrolyze the protein–carbohydrate linkage, peptide N-glycanase and glycosylasparaginase and their mechanism. We then focus on the biosynthesis of O-linked glycoproteins and glycolipids and in particular on the enzymes that hydrolyze the interglycosidic linkages in these, the glycosidases. Some important mechanism-based glycosidase inhibitors that form a covalent bond with the targeted enzyme(s), their corresponding ABPs and their application to study this class of enzymes are highlighted. Finally, alternative pathways for degradation of glycoconjugates and an ABP-based strategy to study these will be discussed.

Graphical abstract: Irreversible inhibitors and activity-based probes as research tools in chemical glycobiology

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 05 Apr 2011, accepted on 03 Jun 2011 and first published on 06 Jun 2011


Article type: Perspective
DOI: 10.1039/C1OB05531C
Citation: Org. Biomol. Chem., 2011,9, 5908-5926
  •   Request permissions

    Irreversible inhibitors and activity-based probes as research tools in chemical glycobiology

    M. D. Witte, G. A. van der Marel, J. M. F. G. Aerts and H. S. Overkleeft, Org. Biomol. Chem., 2011, 9, 5908
    DOI: 10.1039/C1OB05531C

Search articles by author