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Issue 13, 2011
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Photochemically amplified detection of molecular recognition events: an ultra-sensitive fluorescence turn-off binding assay

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Abstract

Amplified fluorescence quenching methodology based on massive autocatalytic photo-unmasking of a dual function sensitizer–quencher is developed and adopted for photoassisted ultra-sensitive detection of molecular recognition events. The resulting binding assay, based on a molecular recognition-triggered photo-amplified cascade with concomitant decrease of fluorescence is validated with the biotin–avidin pair, achieving attomolar detection.

Graphical abstract: Photochemically amplified detection of molecular recognition events: an ultra-sensitive fluorescence turn-off binding assay

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Publication details

The article was received on 23 Feb 2011, accepted on 09 May 2011 and first published on 09 May 2011


Article type: Communication
DOI: 10.1039/C1OB05289F
Citation: Org. Biomol. Chem., 2011,9, 4752-4755
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    Photochemically amplified detection of molecular recognition events: an ultra-sensitive fluorescence turn-off binding assay

    T. P. Gustafson, G. A. Metzel and A. G. Kutateladze, Org. Biomol. Chem., 2011, 9, 4752
    DOI: 10.1039/C1OB05289F

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