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Issue 10, 2011
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Energy transfer cassettes in silica nanoparticles target intracellular organelles

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Abstract

Lipophilic energy transfer cassettes like 1 and 2 are more conveniently synthesized than the corresponding hydrophilic compounds, but they are not easily used in aqueous media. To overcome the latter issue, cassettes 1 and 2 were separately encapsulated in silica nanoparticles (ca. 22 nm) which freely disperse in aqueous media. Photophysical properties of the encapsulated dyes 1SiO2 and 2SiO2 were recorded. The nanoparticles 1SiO2 permeated into Clone 9 rat liver cells and targeted only the ER. A high degree of energy transfer was observed in this organelle such that most of the light fluoresced from the acceptor part, i.e. the particles appeared red. Silica nanoparticles 2SiO2 also permeated into Clone 9 rat liver cells and they targeted mitochondria but were also observed in endocytic vesicles (lysosomes or endosomes). In these organelles they fluoresced red and red/green respectively. Thus the cargo inside the nanoparticles influences where they localize in cells, and the environment of the nanoparticles in the cells changes the fluorescent properties of the encapsulated dyes. Neither of these findings were anticipated given that silica nanoparticles of this type are generally considered to be non-porous.

Graphical abstract: Energy transfer cassettes in silica nanoparticles target intracellular organelles

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Publication details

The article was received on 01 Nov 2010, accepted on 03 Mar 2011 and first published on 03 Mar 2011


Article type: Paper
DOI: 10.1039/C0OB00967A
Citation: Org. Biomol. Chem., 2011,9, 3871-3877
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    Energy transfer cassettes in silica nanoparticles target intracellular organelles

    J. Jose, A. Loudet, Y. Ueno, L. Wu, H. Chen, D. H. Son, R. Barhoumi, R. Burghardt and K. Burgess, Org. Biomol. Chem., 2011, 9, 3871
    DOI: 10.1039/C0OB00967A

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