Covalent immobilization of active lysozyme on Si/glass surface using alkoxy Fischer carbene complex on SAM

Piyali Dutta; Namrata Ray; Sarita Roy; Anjan Kr. Dasgupta; Othman Bouloussa; Amitabha Sarkar

doi:10.1039/C0OB00798F

Covalent immobilization of active lysozyme on Si/glass surface using alkoxy Fischer carbene complex on SAM

Piyali Dutta,^a Namrata Ray,^a Sarita Roy,^b Anjan Kr. Dasgupta,^b Othman Bouloussa^c and Amitabha Sarkar*^a

* Corresponding authors

^a Department of Organic Chemistry, Indian Association for the Cultivation of Science, Kolkata-700032, India
E-mail: ocas@iacs.res.in
Fax: +91 332473280
Tel: +91 3324734971

^b Department of Biochemistry, University of Calcutta, 35, Ballygunge Circular Road, Kolkata-700 019, India

^c Institut Curie, Laboratoire Physico-Chimie Curie (UMR CNRS 168), 26 rue d'Ulm, 75248 Paris cedex 05, France

Abstract

A cross-metathesis reaction between an alkene terminated self-assembled monolayer (SAM) on glass/Si wafer and an alkene tethered Fischer carbene complex yielded a functionalized surface. Rapid aminolysis of the Fischer carbene moieties permit efficient anchoring of amine containing molecules on such a surface. Attachment of 1-pyrenemethylamine was thus monitored by ATR-IR spectroscopy and fluorescence microscopy. Similarly, BSA and lysozyme were individually grafted to such Fischer carbene modified surfaces using their pendant lysine residues. It has been demonstrated that the anchored lysozyme retains its bactericidal property.

Supplementary files

Publication details

The article was received on 29 Sep 2010, accepted on 05 May 2011 and first published on 31 May 2011

Article type: Paper

DOI: 10.1039/C0OB00798F

Citation: Org. Biomol. Chem., 2011,9, 5123-5128

Request permissions
Covalent immobilization of active lysozyme on Si/glass surface using alkoxy Fischer carbene complex on SAM

P. Dutta, N. Ray, S. Roy, A. Kr. Dasgupta, O. Bouloussa and A. Sarkar, Org. Biomol. Chem., 2011, 9, 5123
DOI: 10.1039/C0OB00798F
If you are not the author of this article and you wish to reproduce material from it in a third party non-RSC publication you must formally request permission using RightsLink. Go to our Instructions for using RightsLink page for details.

Authors contributing to RSC publications (journal articles, books or book chapters) do not need to formally request permission to reproduce material contained in this article provided that the correct acknowledgement is given with the reproduced material.

Reproduced material should be attributed as follows:
For reproduction of material from NJC:
Reproduced from Ref. XX with permission from the Centre National de la Recherche Scientifique (CNRS) and The Royal Society of Chemistry.

For reproduction of material from PCCP:
Reproduced from Ref. XX with permission from the PCCP Owner Societies.

For reproduction of material from PPS:
Reproduced from Ref. XX with permission from the European Society for Photobiology, the European Photochemistry Association, and The Royal Society of Chemistry.

For reproduction of material from all other RSC journals and books:
Reproduced from Ref. XX with permission from The Royal Society of Chemistry.
If the material has been adapted instead of reproduced from the original RSC publication "Reproduced from" can be substituted with "Adapted from".

In all cases the Ref. XX is the XXth reference in the list of references.

If you are the author of this article you do not need to formally request permission to reproduce figures, diagrams etc. contained in this article in third party publications or in a thesis or dissertation provided that the correct acknowledgement is given with the reproduced material.

Reproduced material should be attributed as follows:
For reproduction of material from NJC:
[Original citation] - Reproduced by permission of The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC

For reproduction of material from PCCP:
[Original citation] - Reproduced by permission of the PCCP Owner Societies

For reproduction of material from PPS:
[Original citation] - Reproduced by permission of The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC

For reproduction of material from all other RSC journals:
[Original citation] - Reproduced by permission of The Royal Society of Chemistry
If you are the author of this article you still need to obtain permission to reproduce the whole article in a third party publication with the exception of reproduction of the whole article in a thesis or dissertation.

Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Organic & Biomolecular Chemistry

Covalent immobilization of active lysozyme on Si/glass surface using alkoxy Fischer carbene complex on SAM

Abstract

Supplementary files

Publication details

Covalent immobilization of active lysozyme on Si/glass surface using alkoxy Fischer carbene complex on SAM

Search articles by author

Spotlight

Advertisements