The switch-on luminescence sensing of histidine-rich proteins in solution: a further application of a Cu2+ ligand

Bin Wang; Yang Gao; Hong-Wei Li; Zhi-Peng Hu; Yuqing Wu

doi:10.1039/C1OB05372H

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Organic & Biomolecular Chemistry

Issue 11, 2011

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The switch-on luminescence sensing of histidine-rich proteins in solution: a further application of a Cu²⁺ ligand

Bin Wang , Yang Gao , Hong-Wei Li , Zhi-Peng Hu and Yuqing Wu

Org. Biomol. Chem., 2011,9, 4032-4034

DOI: 10.1039/C1OB05372H
Received 10 Mar 2011, Accepted 12 Apr 2011
First published on the web 12 Apr 2011

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Abstract
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A new probe/Cu²⁺ complex for the detection of his-tagged protein has been developed, based on an improved probe, Dansyl-Gly-Py (1), by closely mimicing the structure of a peptide, ATCUN. In aqueous solution, 1/Cu²⁺ has good selectivity to histidine and cysteine, and further can detect histidine-rich protein by releasing the quenched fluorescence of 1.