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Issue 10, 2011
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Enhanced cell uptake via non-covalent decollation of a single-walled carbon nanotube-DNA hybrid with polyethylene glycol-grafted poly(L-lysine) labeled with an Alexa-dye and its efficient uptake in a cancer cell

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Abstract

The use of single-walled carbon nanotubes (SWNTs) for biomedical applications is a promising approach due to their unique outer optical stimuli response properties, such as a photothermal response triggered by near-IR laser irradiation. The challenging task in order to realize such applications is to render the SWNTs biocompatible. For this purpose, the stable and homogeneous functionalization of the SWNTs with a molecule carrying a biocompatible group is very important. Here, we describe the design and synthesis of a polyanionic SWNT/DNA hybrid combined with a cationic poly(L-lysine) grafted by polyethylene glycol (PLL-g-PEG) to provide a supramolecular SWNT assembly. A titration experiment revealed that the assembly undergoes an approximately 1 : 1 reaction of the SWNT/DNA with PLL-g-PEG. We also found that SWNT/DNA is coated with PLL-g-PEG very homogeneously that avoids the non-specific binding of proteins on the SWNT surface. The experiment using the obtained supramolecular hybrid was carried out in vitro and a dramatic enhancement in the cell uptake efficiency compared to that of the SWNT/DNA hybrid without PLL-g-PEG was found.

Graphical abstract: Enhanced cell uptake via non-covalent decollation of a single-walled carbon nanotube-DNA hybrid with polyethylene glycol-grafted poly(l-lysine) labeled with an Alexa-dye and its efficient uptake in a cancer cell

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Publication details

The article was received on 17 Jun 2011, accepted on 12 Aug 2011 and first published on 20 Sep 2011


Article type: Paper
DOI: 10.1039/C1NR10635J
Citation: Nanoscale, 2011,3, 4352-4358
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    Enhanced cell uptake via non-covalent decollation of a single-walled carbon nanotube-DNA hybrid with polyethylene glycol-grafted poly(L-lysine) labeled with an Alexa-dye and its efficient uptake in a cancer cell

    T. Fujigaya, Y. Yamamoto, A. Kano, A. Maruyama and N. Nakashima, Nanoscale, 2011, 3, 4352
    DOI: 10.1039/C1NR10635J

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