Jump to main content
Jump to site search

Issue 5, 2011
Previous Article Next Article

Specific effects of surface carboxyl groups on anionic polystyrene particles in their interactions with mesenchymal stem cells

Author affiliations

Abstract

Nanoparticle uptake by living cells is governed by chemical interactions between functional groups on the nanoparticle as well as the receptors on cell surfaces. Here we have investigated the uptake of anionic polystyrene (PS) nanoparticles of ∼100 nm diameter by mesenchymal stem cells (MSCs) using spinning-disk confocal optical microscopy combined with a quantitative analysis of the fluorescence images. Two types of anionic PS nanoparticles with essentially identical sizes and ζ-potentials were employed in this study, carboxyl-functionalized nanoparticles (CPS) and plain PS nanoparticles, both coated with anionic detergent for stabilization. CPS nanoparticles were observed to internalize more rapidly and accumulate to a much higher level than plain PS nanoparticles. The relative importance of different uptake mechanisms for the two types of nanoparticles was investigated by using specific inhibitors. CPS nanoparticles were internalized mainly via the clathrin-mediated mechanism, whereas plain PS nanoparticles mainly utilized the macropinocytosis pathway. The pronounced difference in the internalization behavior of CPS and plain PS nanoparticles points to a specific interaction of the carboxyl group with receptors on the cell surface.

Graphical abstract: Specific effects of surface carboxyl groups on anionic polystyrene particles in their interactions with mesenchymal stem cells

Back to tab navigation

Publication details

The article was received on 05 Dec 2010, accepted on 18 Feb 2011 and first published on 15 Mar 2011


Article type: Paper
DOI: 10.1039/C0NR00944J
Citation: Nanoscale, 2011,3, 2028-2035
  •   Request permissions

    Specific effects of surface carboxyl groups on anionic polystyrene particles in their interactions with mesenchymal stem cells

    X. Jiang, A. Musyanovych, C. Röcker, K. Landfester, V. Mailänder and G. U. Nienhaus, Nanoscale, 2011, 3, 2028
    DOI: 10.1039/C0NR00944J

Search articles by author

Spotlight

Advertisements