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Issue 3, 2011
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Magnetic CoPt nanoparticles as MRI contrast agent for transplanted neural stem cells detection

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Abstract

Neural stem cells (NSCs) exhibit features that make them suitable candidates for stem cell replacement therapy and spinal cord reconstruction. Magnetic resonance imaging (MRI) offers the potential to track cellsin vivo using innovative approaches to cell labeling and image acquisition. In this study, experiments were carried out to optimize the loading condition of magnetic CoPt hollow nanoparticles (CoPt NPs) into neural stem cells and to define appropriate MRI parameters. Both cell viability and multipotency analysis showed that CoPt NPs at a concentration of 16 µg ml−1 reduced T2 relaxation times in labeled rat NSCs, producing greater contrast on spin echo acquisitions at 4.7 T, yet did not affect cell viability and in vitro differentiation potential compared to controls. After optimizing nanoparticle loading concentrations and labeled cell numbers for MRI detection, CoPt-loaded NSCs were transplanted into organotypic spinal cord slices. The results showed that MRI could efficiently detect low numbers of CoPt-labeled NSCs with the enhanced image contrast. Our study demonstrated that MRI of grafted NSCs labeled with CoPt NPs is a useful tool to evaluate organotypic spinal cord slice models and has potential applications in other biological systems.

Graphical abstract: Magnetic CoPt nanoparticles as MRI contrast agent for transplanted neural stem cells detection

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Publication details

The article was received on 09 Nov 2010, accepted on 06 Dec 2010 and first published on 04 Feb 2011


Article type: Paper
DOI: 10.1039/C0NR00846J
Citation: Nanoscale, 2011,3, 977-984
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    Magnetic CoPt nanoparticles as MRI contrast agent for transplanted neural stem cells detection

    X. Meng, H. C. Seton, L. T. Lu, I. A. Prior, N. T. K. Thanh and B. Song, Nanoscale, 2011, 3, 977
    DOI: 10.1039/C0NR00846J

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