Magnetic CoPt nanoparticles as MRI contrast agent for transplanted neural stem cells detection

Xiaoting Meng; Hugh C. Seton; Le T. Lu; Ian A. Prior; Nguyen T. K. Thanh; Bing Song

doi:10.1039/C0NR00846J

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Nanoscale

Issue 3, 2011

A new peer reviewed journal publishing experimental and theoretical work across the breadth of nanoscience and nanotechnology

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Magnetic CoPt nanoparticles as MRI contrast agent for transplanted neural stem cells detection

Xiaoting Meng,^a Hugh C. Seton,^b Le T. Lu,^c Ian A. Prior,^d Nguyen T. K. Thanh*^e^f and Bing Song*^a^b

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School of Dentistry, Cardiff Institute of Tissue Engineering & Repair, Cardiff University, Cardiff, UK
E-mail: SongB3@cardiff.ac.uk ;
Fax: +44 (0)29-20748168 ;
Tel: +44 (0)29-20744182

School of Medical Sciences, University of Aberdeen, UK

Department of Chemistry, University of Liverpool, UK

Physiological Laboratory, University of Liverpool, Liverpool, UK

The Davy-Faraday Research Laboratory, The Royal Institution of Great Britain, 21 Albemarle Street, London, UK
E-mail: ntk.thanh@ucl.ac.uk ;
Fax: +44 (0)207-670-2920 ;
Tel: +44 (0)207-491-6509

Department of Physics & Astronomy, University College London, Gower Street, London, UK

Nanoscale, 2011,3, 977-984

DOI: 10.1039/C0NR00846J
Received 09 Nov 2010, Accepted 06 Dec 2010
First published online 04 Feb 2011

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Neural stem cells (NSCs) exhibit features that make them suitable candidates for stem cell replacement therapy and spinal cord reconstruction. Magnetic resonance imaging (MRI) offers the potential to track cells in vivo using innovative approaches to cell labeling and image acquisition. In this study, experiments were carried out to optimize the loading condition of magnetic CoPt hollow nanoparticles (CoPt NPs) into neural stem cells and to define appropriate MRI parameters. Both cell viability and multipotency analysis showed that CoPt NPs at a concentration of 16 µg ml⁻¹ reduced T₂ relaxation times in labeled rat NSCs, producing greater contrast on spin echo acquisitions at 4.7 T, yet did not affect cell viability and in vitro differentiation potential compared to controls. After optimizing nanoparticle loading concentrations and labeled cell numbers for MRI detection, CoPt-loaded NSCs were transplanted into organotypic spinal cord slices. The results showed that MRI could efficiently detect low numbers of CoPt-labeled NSCs with the enhanced image contrast. Our study demonstrated that MRI of grafted NSCs labeled with CoPt NPs is a useful tool to evaluate organotypic spinal cord slice models and has potential applications in other biological systems.

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Xiaoting Meng
Hugh C. Seton
Le T. Lu
Ian A. Prior
Nguyen T. K. Thanh
Bing Song

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