Combination of PAGE and LA-ICP-MS as an analytical workflow in metallomics: state of the art, new quantification strategies, advantages and limitations

Alessandra Sussulini; Johanna Sabine Becker

doi:10.1039/C1MT00116G

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Metallomics

Issue 12, 2011

A new journal covering the research fields related to biometals

Impact Factor 3.902 12 Issues per Year Indexed in MEDLINE

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Combination of PAGE and LA-ICP-MS as an analytical workflow in metallomics: state of the art, new quantification strategies, advantages and limitations

Alessandra Sussulini*^a and Johanna Sabine Becker^a

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Central Division of Analytical Chemistry, Forschungszentrum Jülich, J, Germany
E-mail: a.sussulini@fz-juelich.de

Metallomics, 2011,3, 1271-1279

DOI: 10.1039/C1MT00116G
Received 26 Jul 2011, Accepted 10 Oct 2011
First published online 24 Oct 2011

This article is part of themed collection: Third International Symposium on Metallomics, Münster, Germany 2011

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Abstract
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Metallomics (more specifically, metalloproteomics) is an emerging field that encompasses the role, uptake, transport and storage of trace metals, which are essential to preserve the functions of proteins within a biological system. The current strategies for metal-binding and metalloprotein analysis based on the combination of polyacrylamide gel electrophoresis (PAGE) and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) are discussed in this review. The advantages, limitations and the most recently developed and applied quantification approaches for this methodology are also described.

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