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Issue 7, 2011
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Alterations in urinary metabolites due to unilateral ureteral obstruction in a rodent model

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Abstract

Urinary tract obstruction (UTO) results in renal compensatory mechanisms and may progress to irrecoverable functional loss and histologic alterations. The pathophysiology of this progression is poorly understood. We identified urinary metabolite alterations in a rodent model of partial and complete UTO using 1H nuclear magnetic resonance (1H-NMR) spectroscopy. Principal component analysis (PCA) was used for classification and discovery of differentiating metabolites. UTO was associated with elevated urinary levels of alanine, succinate, dimethylglycine (DMG), creatinine, taurine, choline-like compounds, hippurate, and lactate. Decreased urinary levels of 2-oxoglutarate and citrate were noted. The patterns of alteration in partial and complete UTO were similar except that an absence of elevated urinary osmolytes (DMG and hippurate) was noted in complete UTO. This pattern of metabolite alteration indicates impaired oxidative metabolism of the mitochondria in renal proximal tubules and production of renal protective osmolytes by the medulla. Decreased production of osmolytes in complete obstruction better elucidates the pathophysiology of progression from renal compensatory mechanisms to irrecoverable changes. Further confirmation of these potential biomarkers in children with UTO is necessary.

Graphical abstract: Alterations in urinary metabolites due to unilateral ureteral obstruction in a rodent model

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Publication details

The article was received on 25 Feb 2011, accepted on 19 Apr 2011 and first published on 06 May 2011


Article type: Paper
DOI: 10.1039/C1MB05080J
Citation: Mol. BioSyst., 2011,7, 2181-2188
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    Alterations in urinary metabolites due to unilateral ureteral obstruction in a rodent model

    D. L. MacLellan, D. Mataija, A. Doucette, W. Huang, C. Langlois, G. Trottier, I. W. Burton, J. A. Walter and T. K. Karakach, Mol. BioSyst., 2011, 7, 2181
    DOI: 10.1039/C1MB05080J

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