Jump to main content
Jump to site search

Issue 4, 2011
Previous Article Next Article

Rapid characterization and engineering of natural product biosynthetic pathways viaDNA assembler

Author affiliations

Abstract

We report a synthetic biology strategy for rapid genetic manipulation of natural product biosynthetic pathways. Based on DNA assembler, this method synthesizes the entire expression vector containing the target biosynthetic pathway and the genetic elements required for DNA maintenance and replication in various hosts in a single-step manner through yeast homologous recombination, offering unprecedented flexibility and versatility in pathway manipulations.

Graphical abstract: Rapid characterization and engineering of natural product biosynthetic pathways viaDNA assembler

Back to tab navigation

Supplementary files

Publication details

The article was received on 18 Dec 2010, accepted on 31 Jan 2011 and first published on 16 Feb 2011


Article type: Communication
DOI: 10.1039/C0MB00338G
Citation: Mol. BioSyst., 2011,7, 1056-1059
  •   Request permissions

    Rapid characterization and engineering of natural product biosynthetic pathways viaDNA assembler

    Z. Shao, Y. Luo and H. Zhao, Mol. BioSyst., 2011, 7, 1056
    DOI: 10.1039/C0MB00338G

Search articles by author

Spotlight

Advertisements