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Issue 4, 2011
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Rapid characterization and engineering of natural product biosynthetic pathways viaDNA assembler

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Abstract

We report a synthetic biology strategy for rapid genetic manipulation of natural product biosynthetic pathways. Based on DNA assembler, this method synthesizes the entire expression vector containing the target biosynthetic pathway and the genetic elements required for DNA maintenance and replication in various hosts in a single-step manner through yeast homologous recombination, offering unprecedented flexibility and versatility in pathway manipulations.

Graphical abstract: Rapid characterization and engineering of natural product biosynthetic pathways viaDNA assembler

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Publication details

The article was received on 18 Dec 2010, accepted on 31 Jan 2011 and first published on 16 Feb 2011


Article type: Communication
DOI: 10.1039/C0MB00338G
Citation: Mol. BioSyst., 2011,7, 1056-1059
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    Rapid characterization and engineering of natural product biosynthetic pathways viaDNA assembler

    Z. Shao, Y. Luo and H. Zhao, Mol. BioSyst., 2011, 7, 1056
    DOI: 10.1039/C0MB00338G

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