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Issue 5, 2011
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Microarray-based target identification using drug hypersensitive fission yeast expressing ORFeome

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Abstract

Identification of the cellular target of small molecules is a major challenge to developing biological tools and drug leads. Here we report a novel microarray-based system for identification of the target or the target pathway of small molecules using a set of drug-hypersensitive fission yeast strains that collectively overexpress each gene in the open reading frame-ome. The major advantage of this method is that it provides genome-wide interrogation but requires a relatively small amount of the test compound. Using this system, we identified 28 genes linked to etoposide sensitivity, which included genes for the drug target topoisomerase II and other plausible factors that regulate etoposide tolerance. Thus, our approach can accelerate the process of target identification of small molecules, which has the potential to reveal highly conserved genes of clinical relevance.

Graphical abstract: Microarray-based target identification using drug hypersensitive fission yeast expressing ORFeome

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Publication details

The article was received on 13 Dec 2010, accepted on 24 Jan 2011 and first published on 22 Feb 2011


Article type: Paper
DOI: 10.1039/C0MB00326C
Citation: Mol. BioSyst., 2011,7, 1463-1472
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    Microarray-based target identification using drug hypersensitive fission yeast expressing ORFeome

    Y. Arita, S. Nishimura, A. Matsuyama, Y. Yashiroda, T. Usui, C. Boone and M. Yoshida, Mol. BioSyst., 2011, 7, 1463
    DOI: 10.1039/C0MB00326C

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