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Issue 2, 2011
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Targeted proteomics by selected reaction monitoring mass spectrometry: applications to systems biology and biomarker discovery

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Abstract

Mass Spectrometry-based proteomics is now considered a relatively established strategy for protein analysis, ranging from global expression profiling to the identification of protein complexes and specific post-translational modifications. Recently, Selected Reaction Monitoring Mass Spectrometry (SRM-MS) has become increasingly popular in proteome research for the targeted quantification of proteins and post-translational modifications. Using triple quadrupole instrumentation (QqQ), specific analyte molecules are targeted in a data-directed mode. Used routinely for the quantitative analysis of small molecular compounds for at least three decades, the technology is now experiencing broadened application in the proteomics community. In the current review, we will provide a detailed summary of current developments in targeted proteomics, including some of the recent applications to biological research and biomarker discovery.

Graphical abstract: Targeted proteomics by selected reaction monitoring mass spectrometry: applications to systems biology and biomarker discovery

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Publication details

The article was received on 13 Aug 2010, accepted on 22 Sep 2010 and first published on 25 Oct 2010


Article type: Review Article
DOI: 10.1039/C0MB00159G
Citation: Mol. BioSyst., 2011,7, 292-303
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    Targeted proteomics by selected reaction monitoring mass spectrometry: applications to systems biology and biomarker discovery

    S. Elschenbroich and T. Kislinger, Mol. BioSyst., 2011, 7, 292
    DOI: 10.1039/C0MB00159G

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