Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 2, 2011
Previous Article Next Article

Calorie restriction up-regulates iron and copper transport genes in Saccharomyces cerevisiae

Author affiliations

Abstract

Calorie restriction (CR) is a non genetic intervention, known to confer longevity benefits across the various phyla from unicellular yeast to mammals. CR also invokes homeostatic responses similar to stress, however the sequence of molecular events leading to longevity is still illusive. In this study, we analysed the whole genome gene expression profile in response to CR, mutations mimicking CR, heat shock and H2O2 from a gene ontology perspective. Our analysis revealed that mitochondrion is a common hub in the gene expression programme under these conditions and the electron transport chain (ETC) is a major player. Consequently the genes involved in the metal ion transport were also significantly up-regulated. We confirmed the results of the in silico analysis using quantitative real time PCR which showed up-regulation of genes involved in respiration and transport of iron and copper. The promoter activity of one of the representative genes, FET3, was also found to be higher upon calorie restriction. Altogether, our results indicate that upon calorie restriction the levels of iron and copper fall in cells, which elicits a transcriptional response up-regulating the genes involved in their uptake to maintain cellular homeostasis.

Graphical abstract: Calorie restriction up-regulates iron and copper transport genes in Saccharomyces cerevisiae

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 07 Jul 2010, accepted on 07 Sep 2010 and first published on 28 Oct 2010


Article type: Paper
DOI: 10.1039/C0MB00084A
Citation: Mol. BioSyst., 2011,7, 394-402
  •   Request permissions

    Calorie restriction up-regulates iron and copper transport genes in Saccharomyces cerevisiae

    P. K. Sharma, N. Mittal, S. Deswal and N. Roy, Mol. BioSyst., 2011, 7, 394
    DOI: 10.1039/C0MB00084A

Search articles by author