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Issue 23, 2011
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Rapid purification of cell encapsulated hydrogel beads from oil phase to aqueous phase in a microfluidic device

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Abstract

In this paper, we demonstrate a new type of microfluidic chip that can realize continuous-flow purification of hydrogel beads from a carrier oil into aqueous solution by using a laminar-like oil/water interface. The microfluidic chip is composed by two functional components: (1) a flow-focusing bead generation module that can control size and shape of beads, (2) a bead extraction module capable of purifying hydrogel beads from oil into aqueous solution. This module is featured with large branch channels on one side and small ones on the opposite side. Water is continuously infused into the bead extraction module through the large branch channels, resulting in a laminar-like oil/water interface between the branch junctions. Simulation and experimental data show that the efficiency of oil depletion is determined by the relative flow rates between infused water and carrier oil. By using such a microfluidic device, viable cells (HCT116, colon cancer cell line) can be encapsulated in the hydrogel beads and purified into a cell culture media. Significantly improved cell viability was achieved compared to that observed by conventional bead purification approaches. This facile microfluidic chip could be a promising candidate for sample treatment in lab-on-a-chip applications.

Graphical abstract: Rapid purification of cell encapsulated hydrogel beads from oil phase to aqueous phase in a microfluidic device

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Publication details

The article was received on 07 Jun 2011, accepted on 09 Sep 2011 and first published on 19 Oct 2011


Article type: Technical Note
DOI: 10.1039/C1LC20494G
Citation: Lab Chip, 2011,11, 4117-4121
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    Rapid purification of cell encapsulated hydrogel beads from oil phase to aqueous phase in a microfluidic device

    Y. Deng, N. Zhang, L. Zhao, X. Yu, X. Ji, W. Liu, S. Guo, K. Liu and X. Zhao, Lab Chip, 2011, 11, 4117
    DOI: 10.1039/C1LC20494G

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