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Issue 19, 2011
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Separation of platelets from whole blood using standing surface acoustic waves in a microchannel

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Abstract

Platelet separation from blood is essential for biochemical analyses and clinical diagnosis. In this article, we propose a method to separate platelets from undiluted whole blood using standing surface acoustic waves (SSAWs) in a microfluidic device. A polydimethylsiloxane (PDMS) microfluidic channel was fabricated and integrated with interdigitated transducer (IDT) electrodes patterned on a piezoelectric substrate. To avoid shear-induced activation of platelets, the blood sample flow was hydrodynamically focused by introducing sheath flow from two side-inlets and pressure nodes were designed to locate at side walls. By means of flow cytometric analysis, the RBC clearance ratio from whole blood was found to be over 99% and the purity of platelets was close to 98%. Conclusively, the present technique using SSAWs can directly separate platelets from undiluted whole blood with higher purity than other methods.

Graphical abstract: Separation of platelets from whole blood using standing surface acoustic waves in a microchannel

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Publication details

The article was received on 25 Apr 2011, accepted on 26 Jul 2011 and first published on 15 Aug 2011


Article type: Technical Note
DOI: 10.1039/C1LC20346K
Citation: Lab Chip, 2011,11, 3361-3364
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    Separation of platelets from whole blood using standing surface acoustic waves in a microchannel

    J. Nam, H. Lim, D. Kim and S. Shin, Lab Chip, 2011, 11, 3361
    DOI: 10.1039/C1LC20346K

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