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Issue 17, 2011
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Histone modification analysis by chromatin immunoprecipitation from a low number of cells on a microfluidic platform

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Abstract

Histone modifications are important epigenetic mechanisms involved in eukaryotic gene regulation. Chromatin immunoprecipitation (ChIP) assay serves as the primary technique to characterize the genomic locations associated with histone modifications. However, traditional tube-based ChIP assays rely on large numbers of cells as well as laborious and time-consuming procedures. Here we demonstrate a novel microfluidics-based native ChIP assay which dramatically reduces the required cell number and the assay time by conducting cell collection, lysis, chromatin fragmentation, immunoprecipitation, and washing on a microchip. Coupled with real-time PCR, our assay permits the analysis of histone modifications from as few as ∼50 cells within 8.5 h. We envision that our method will provide a new approach for the analysis of epigenetic regulations and protein–DNA interactions in general, based on scarce cell samples such as those derived from animals and patients.

Graphical abstract: Histone modification analysis by chromatin immunoprecipitation from a low number of cells on a microfluidic platform

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Publication details

The article was received on 25 Mar 2011, accepted on 24 Jun 2011 and first published on 13 Jul 2011


Article type: Paper
DOI: 10.1039/C1LC20253G
Citation: Lab Chip, 2011,11, 2842-2848
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    Histone modification analysis by chromatin immunoprecipitation from a low number of cells on a microfluidic platform

    T. Geng, N. Bao, M. D. Litt, T. G. Glaros, L. Li and C. Lu, Lab Chip, 2011, 11, 2842
    DOI: 10.1039/C1LC20253G

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