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Issue 7, 2011
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A first step towards practical single cell proteomics: a microfluidic antibody capture chip with TIRF detection

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Abstract

We have developed a generic platform to undertake the analysis of protein copy number from single cells. The approach described here is ‘all-optical’ whereby single cells are manipulated into separate analysis chambers using an optical trap; single cells are lysed by a shock wave caused by laser-induced microcavitation, and the protein released from a single cell is measured by total internal reflection microscopy as it is bound to micro-printed antibody spots within the device. The platform was tested using GFP transfected cells and the relative precision of the measurement method was determined to be 88%. Single cell measurements were also made on a breast cancer cell line to measure the relative levels of unlabelled human tumour suppressor protein p53 using a chip incorporating an antibody sandwich assay format. These results suggest that this is a viable method for measuring relative protein levels in single cells.

Graphical abstract: A first step towards practical single cell proteomics: a microfluidic antibody capture chip with TIRF detection

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Publication details

The article was received on 19 Nov 2010, accepted on 27 Jan 2011 and first published on 23 Feb 2011


Article type: Paper
DOI: 10.1039/C0LC00613K
Citation: Lab Chip, 2011,11, 1256-1261
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    A first step towards practical single cell proteomics: a microfluidic antibody capture chip with TIRF detection

    A. Salehi-Reyhani, J. Kaplinsky, E. Burgin, M. Novakova, A. J. deMello, R. H. Templer, P. Parker, M. A. A. Neil, O. Ces, P. French, K. R. Willison and D. Klug, Lab Chip, 2011, 11, 1256
    DOI: 10.1039/C0LC00613K

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