Jump to main content
Jump to site search

Issue 8, 2011
Previous Article Next Article

Tumour cell identification by means of Raman spectroscopy in combination with optical traps and microfluidic environments

Author affiliations

Abstract

Raman spectroscopy has been recognized to be a powerful tool for label-free discrimination of cells. Sampling methods are under development to utilize the unique capabilities to identify cells in body fluids such as saliva, urine or blood. The current study applied optical traps in combination with Raman spectroscopy to acquire spectra of single cells in microfluidic glass channels. Optical traps were realized by two 1070 nm single mode fibre lasers. Microflows were controlled by a syringe pump system. A novel microfluidic glass chip was designed to inject single cells, modify the flow speed, accommodate the laser fibres and sort cells after Raman based identification. Whereas the integrated microchip setup used 514 nm for excitation of Raman spectra, a quartz capillary setup excited spectra with 785 nm laser wavelength. Classification models were trained using linear discriminant analysis to differentiate erythrocytes, leukocytes, acute myeloid leukaemia cells (OCI-AML3), and breast tumour cells BT-20 and MCF-7 with accuracies that are comparable with previous Raman experiments of dried cells and fixed cells in a Petri dish. Implementation into microfluidic environments enables a high degree of automation that is required to improve the throughput of the approach for Raman activated cell sorting.

Graphical abstract: Tumour cell identification by means of Raman spectroscopy in combination with optical traps and microfluidic environments

Back to tab navigation

Publication details

The article was received on 18 Nov 2010, accepted on 03 Feb 2011 and first published on 22 Feb 2011


Article type: Paper
DOI: 10.1039/C0LC00612B
Citation: Lab Chip, 2011,11, 1484-1490
  •   Request permissions

    Tumour cell identification by means of Raman spectroscopy in combination with optical traps and microfluidic environments

    S. Dochow, C. Krafft, U. Neugebauer, T. Bocklitz, T. Henkel, G. Mayer, J. Albert and J. Popp, Lab Chip, 2011, 11, 1484
    DOI: 10.1039/C0LC00612B

Search articles by author

Spotlight

Advertisements