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Issue 8, 2011
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Dual-color fluorescence cross-correlation spectroscopy on a planar optofluidic chip

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Abstract

Fluorescence cross-correlation spectroscopy (FCCS) is a highly sensitive fluorescence technique with distinct advantages in many bioanalytical applications involving interaction and binding of multiple components. Due to the use of multiple beams, bulk optical FCCS setups require delicate and complex alignment procedures. We demonstrate the first implementation of dual-color FCCS on a planar, integrated optofluidic chip based on liquid-core waveguides that can guide liquid and light simultaneously. In this configuration, the excitation beams are delivered in predefined locations and automatically aligned within the excitation waveguides. We implement two canonical applications of FCCS in the optofluidic lab-on-chip environment: particle colocalization and binding/dissociation dynamics. Colocalization is demonstrated in the detection and discrimination of single-color and double-color fluorescently labeled nanobeads. FCCS in combination with fluorescence resonance energy transfer (FRET) is used to detect the denaturation process of double-stranded DNA at nanomolar concentration.

Graphical abstract: Dual-color fluorescence cross-correlation spectroscopy on a planar optofluidic chip

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Publication details

The article was received on 12 Sep 2010, accepted on 28 Jan 2011 and first published on 22 Feb 2011


Article type: Paper
DOI: 10.1039/C0LC00401D
Citation: Lab Chip, 2011,11, 1502-1506
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    Dual-color fluorescence cross-correlation spectroscopy on a planar optofluidic chip

    A. Chen, M. M. Eberle, E. J. Lunt, S. Liu, K. Leake, M. I. Rudenko, A. R. Hawkins and H. Schmidt, Lab Chip, 2011, 11, 1502
    DOI: 10.1039/C0LC00401D

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