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Issue 3, 2011
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Microscale 3-D hydrogel scaffold for biomimetic gastrointestinal (GI) tract model

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Abstract

Here we describe a simple and efficient method for fabricating natural and synthetic hydrogels into 3-D geometries with high aspect ratio and curvature. Fabricating soft hydrogels into such shapes using conventional techniques has been extremely difficult. Combination of laser ablation and sacrificial molding technique using calcium alginate minimizes the stress associated with separating the mold from the hydrogel structure, and therefore allows fabrication of complex structures without damaging them. As a demonstration of this technique, we have fabricated a microscale collagen structure mimicking the actual density and size of human intestinal villi. Colon carcinoma cell line, Caco-2 cells, was seeded onto the structure and cultured for 3 weeks until the whole structure was covered, forming finger-like structures mimicking the intestinal villi covered with epithelial cells. This method will enable construction of in vitro tissue models with physiologically realistic geometries at microscale resolutions.

Graphical abstract: Microscale 3-D hydrogel scaffold for biomimetic gastrointestinal (GI) tract model

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Publication details

The article was received on 03 Aug 2010, accepted on 19 Nov 2010 and first published on 15 Dec 2010


Article type: Communication
DOI: 10.1039/C0LC00273A
Citation: Lab Chip, 2011,11, 389-392
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    Microscale 3-D hydrogel scaffold for biomimetic gastrointestinal (GI) tract model

    J. H. Sung, J. Yu, D. Luo, M. L. Shuler and J. C. March, Lab Chip, 2011, 11, 389
    DOI: 10.1039/C0LC00273A

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