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Issue 14, 2011
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Inkjet-like printing of single-cells

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Abstract

Cell sorting and separation techniques are essential tools for cell biology research and for many diagnostic and therapeutic applications. For many of these applications, it is imperative that heterogeneous populations of cells are segregated according to their cell type and that individual cells can be isolated and analysed. We present a novel technique to isolate single cells encapsulated in a picolitre sized droplet that are then deposited by inkjet-like printing at defined locations for downstream genomic analysis. The single-cell-manipulator (SCM) developed for this purpose consists of a dispenser chip to print cells contained in a free flying droplet, a computer vision system to detect single-cells inside the dispenser chip prior to printing, and appropriate automation equipment to print single-cells onto defined locations on a substrate. This technique is spatially dynamic, enabling cell printing on a wide range of commonly used substrates such as microscope slides, membranes and microtiter plates. Demonstration experiments performed using the SCM resulted in a printing efficiency of 87% for polystyrene microbeads of 10 μm size. When the SCM was applied to a cervical cancer cell line (HeLa), a printing efficiency of 87% was observed and a post-SCM cell viability rate of 75% was achieved.

Graphical abstract: Inkjet-like printing of single-cells

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Publication details

The article was received on 01 Mar 2011, accepted on 10 May 2011 and first published on 09 Jun 2011


Article type: Paper
DOI: 10.1039/C1LC20176J
Citation: Lab Chip, 2011,11, 2447-2454
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    Inkjet-like printing of single-cells

    A. Yusof, H. Keegan, C. D. Spillane, O. M. Sheils, C. M. Martin, J. J. O'Leary, R. Zengerle and P. Koltay, Lab Chip, 2011, 11, 2447
    DOI: 10.1039/C1LC20176J

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