Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 5, 2011
Previous Article Next Article

The effects of shear stress on isolated receptor–ligand interactions of Staphylococcus epidermidis and human plasma fibrinogen using molecularly patterned microfluidics

Author affiliations

Abstract

Staphylococcus epidermidis is an opportunistic pathogen that has been implicated in hospital-acquired infections, specifically related to implanted intravascular devices. S. epidermidis adhesion is a mechanism of colonization, leading to pathogenesis. Here we demonstrate an easily fabricated and robust parallel microfluidic platform to investigate the physiologically-relevant effects of fluid shear on S. epidermidis adhesion to human fibrinogen (hFg) with increased experimental throughput. In situ molecular patterning using fluid flow boundaries allows for isolation of the molecular interactions in highly defined shear stress environments, while keeping the device operation simple and reproducible. We characterize two modes of attachment of S. epidermidis to hFg coated surfaces. Single colonies adhere in high fractions at low shear stresses (~1 dyne cm−2) and adhesion decays with increasing shear. However, clusters of bacteria adhere the highest at median wall shear stress (up to 10 dyne cm−2), and adhesion subsequently decays above this critical shear stress. This initial characterization suggests a previously unobserved phenomenon of shear activated cell–cell adhesion in S. epidermidis, which acts to increase the overall attachment strength to hFg. Both of these modes of attachment are dependant upon the presence of intact hFg, indicating that adhesion is resultant from specific molecular recognition between the bacteria and human fibrinogen. This platform provides new insight into complex host–pathogen interactions, and will allow for further investigation of colonization and pathogenesis in more physiologically relevant conditions.

Graphical abstract: The effects of shear stress on isolated receptor–ligand interactions of Staphylococcus epidermidis and human plasma fibrinogen using molecularly patterned microfluidics

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 15 Sep 2010, accepted on 13 Dec 2010 and first published on 20 Jan 2011


Article type: Paper
DOI: 10.1039/C0LC00414F
Citation: Lab Chip, 2011,11, 883-889
  •   Request permissions

    The effects of shear stress on isolated receptor–ligand interactions of Staphylococcus epidermidis and human plasma fibrinogen using molecularly patterned microfluidics

    W. M. Weaver, S. Dharmaraja, V. Milisavljevic and D. Di Carlo, Lab Chip, 2011, 11, 883
    DOI: 10.1039/C0LC00414F

Search articles by author