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Issue 4, 2011
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EIS microfluidic chips for flow immunoassay and ultrasensitive cholera toxin detection

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Abstract

A flow-injection impedimetric immunosensor for the sensitive, direct and label-free detection of cholera toxin is reported. A limit of detection smaller than 10 pM was achieved, a value thousands of times lower than the lethal dose. The developed chips fulfil the requirement of low cost and quick reply of the assay and are expected to enable field screening, prompt diagnosis and medical intervention without the need of specialized personnel and expensive equipment, a perspective of special relevance for use in developing countries. Since the chip layout includes two sensing areas each one with a 2 × 2 sensor array, our biochips can allow statistical or (alternatively) multiplex analysis of biorecognition events between antibodies immobilized on each working electrode and different antigens flowing into the chamber.

Graphical abstract: EIS microfluidic chips for flow immunoassay and ultrasensitive cholera toxin detection

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Publication details

The article was received on 14 Sep 2010, accepted on 29 Oct 2010 and first published on 03 Dec 2010


Article type: Paper
DOI: 10.1039/C0LC00409J
Citation: Lab Chip, 2011,11, 658-663
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    EIS microfluidic chips for flow immunoassay and ultrasensitive cholera toxin detection

    M. S. Chiriacò, E. Primiceri, E. D'Amone, R. E. Ionescu, R. Rinaldi and G. Maruccio, Lab Chip, 2011, 11, 658
    DOI: 10.1039/C0LC00409J

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