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Issue 4, 2011
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Integrated microfluidic platform for the electrochemical detection of breast cancer markers in patient serum samples

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Abstract

A microsystem integrating electrochemical detection for the simultaneous detection of protein markers of breast cancer is reported. The microfluidic platform was realized by high precision milling of polycarbonate sheets and features two well distinguishable sections: a detection zone incorporating the electrode arrays and the fluid storage part. The detection area is divided into separate microfluidic chambers addressing selected electrodes for the measurement of samples and calibrators. The fluidic storage part of the platform consists of five reservoirs to store the reagents and sample, which are interfaced by septa. These reservoirs have the appropriate volume to run a single assay per cartridge and are manually filled. The liquids from the reservoirs are actuated by applying a positive air pressure (i.e.via a programmable syringe pump) through the septa and are driven to the detection zone via two turning valves. The application of the realised platform in the individual and simultaneous electrochemical detection of proteic cancer markers with very low detection limits are demonstrated. The microsystem has also been validated using real patient serum samples and excellent correlation with ELISA results obtained.

Graphical abstract: Integrated microfluidic platform for the electrochemical detection of breast cancer markers in patient serum samples

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Publication details

The article was received on 10 Sep 2010, accepted on 09 Nov 2010 and first published on 01 Dec 2010


Article type: Paper
DOI: 10.1039/C0LC00398K
Citation: Lab Chip, 2011,11, 625-631
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    Integrated microfluidic platform for the electrochemical detection of breast cancer markers in patient serum samples

    A. Fragoso, D. Latta, N. Laboria, F. von Germar, T. E. Hansen-Hagge, W. Kemmner, C. Gärtner, R. Klemm, K. S. Drese and C. K. O'Sullivan, Lab Chip, 2011, 11, 625
    DOI: 10.1039/C0LC00398K

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