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Issue 4, 2011
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Combination of fluid and solid mechanical stresses contribute to cell death and detachment in a microfluidic alveolar model

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Abstract

Studies using this micro-system demonstrated significant morphological differences between alveolar epithelial cells (transformed human alveolar epithelial cell line, A549 and primary murine alveolar epithelial cells, AECs) exposed to combination of solid mechanical and surface-tension stresses (cyclic propagation of air–liquid interface and wall stretch) compared to cell populations exposed solely to cyclic stretch. We have also measured significant differences in both cell death and cell detachment rates in cell monolayers experiencing combination of stresses. This research describes new tools for studying the combined effects of fluid mechanical and solid mechanical stress on alveolar cells. It also highlights the role that surface tension forces may play in the development of clinical pathology, especially under conditions of surfactant dysfunction. The results support the need for further research and improved understanding on techniques to reduce and eliminate fluid stresses in clinical settings.

Graphical abstract: Combination of fluid and solid mechanical stresses contribute to cell death and detachment in a microfluidic alveolar model

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Publication details

The article was received on 28 Jul 2010, accepted on 15 Nov 2010 and first published on 09 Dec 2010


Article type: Paper
DOI: 10.1039/C0LC00251H
Citation: Lab Chip, 2011,11, 609-619
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    Combination of fluid and solid mechanical stresses contribute to cell death and detachment in a microfluidic alveolar model

    N. J. Douville, P. Zamankhan, Y. Tung, R. Li, B. L. Vaughan, C. Tai, J. White, P. J. Christensen, J. B. Grotberg and S. Takayama, Lab Chip, 2011, 11, 609
    DOI: 10.1039/C0LC00251H

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