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Issue 1, 2011
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A printed nanolitre-scale bacterial sensor array

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Abstract

The last decade has witnessed a significant increase in interest in whole-cell biosensors for diverse applications, as well as a rapid and continuous expansion of array technologies. The combination of these two disciplines has yielded the notion of whole-cell array biosensors. We present a potential manifestation of this idea by describing the printing of a whole-cell bacterial bioreporters array. Exploiting natural bacterial tendency to adhere to positively charged abiotic surfaces, we describe immobilization and patterning of bacterial “spots” in the nanolitre volume range by a non-contact robotic printer. We show that the printed Escherichia coli-based sensor bacteria are immobilized on the surface, and retain their viability and biosensing activity for at least 2 months when kept at 4 °C. Immobilization efficiency was improved by manipulating the bacterial genetics (overproducing curli protein), the growth and the printing media (osmotic stress and osmoprotectants) and by a chemical modification of the inanimate surface (self-assembled layers of 3-aminopropyl-triethoxysilane). We suggest that the methodology presented herein may be applicable to the manufacturing of whole-cell sensor arrays for diverse high throughput applications.

Graphical abstract: A printed nanolitre-scale bacterial sensor array

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Publication details

The article was received on 26 Jul 2010, accepted on 01 Oct 2010 and first published on 26 Oct 2010


Article type: Paper
DOI: 10.1039/C0LC00243G
Citation: Lab Chip, 2011,11, 139-146
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    A printed nanolitre-scale bacterial sensor array

    S. Melamed, L. Ceriotti, W. Weigel, F. Rossi, P. Colpo and S. Belkin, Lab Chip, 2011, 11, 139
    DOI: 10.1039/C0LC00243G

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