Jump to main content
Jump to site search

Issue 1, 2011
Previous Article Next Article

A printed nanolitre-scale bacterial sensor array

Author affiliations


The last decade has witnessed a significant increase in interest in whole-cell biosensors for diverse applications, as well as a rapid and continuous expansion of array technologies. The combination of these two disciplines has yielded the notion of whole-cell array biosensors. We present a potential manifestation of this idea by describing the printing of a whole-cell bacterial bioreporters array. Exploiting natural bacterial tendency to adhere to positively charged abiotic surfaces, we describe immobilization and patterning of bacterial “spots” in the nanolitre volume range by a non-contact robotic printer. We show that the printed Escherichia coli-based sensor bacteria are immobilized on the surface, and retain their viability and biosensing activity for at least 2 months when kept at 4 °C. Immobilization efficiency was improved by manipulating the bacterial genetics (overproducing curli protein), the growth and the printing media (osmotic stress and osmoprotectants) and by a chemical modification of the inanimate surface (self-assembled layers of 3-aminopropyl-triethoxysilane). We suggest that the methodology presented herein may be applicable to the manufacturing of whole-cell sensor arrays for diverse high throughput applications.

Graphical abstract: A printed nanolitre-scale bacterial sensor array

Back to tab navigation

Publication details

The article was received on 26 Jul 2010, accepted on 01 Oct 2010 and first published on 26 Oct 2010

Article type: Paper
DOI: 10.1039/C0LC00243G
Citation: Lab Chip, 2011,11, 139-146
  •   Request permissions

    A printed nanolitre-scale bacterial sensor array

    S. Melamed, L. Ceriotti, W. Weigel, F. Rossi, P. Colpo and S. Belkin, Lab Chip, 2011, 11, 139
    DOI: 10.1039/C0LC00243G

Search articles by author