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Issue 44, 2011
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Linker-free covalent thermophilic β-glucosidase functionalized polymeric surfaces

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Abstract

The surface immobilization of enzymes is important for many biomedical and industrial applications; however, the choice of enzyme and immobilization method for best performance is often difficult. This paper demonstrates that Plasma Immersion Ion Implantation (PIII) provides high enzyme coverage and rapid covalent attachment (within the first minute) of incubation, and shows how this can be used to control the composition of the attached layer from an enzyme mixture. The use of a thermophilic enzyme combined with covalent immobilization viaPIII-treatment significantly improves β-glucosidase activity. β-glucosidase is an important rate-limiting enzyme in the production of cellulosic biofuel and is useful in many other industrial applications. β-glucosidase immobilized on the PIII-treated polystyrene surface demonstrated significant improvements in reaction rate and glucose yields compared to the enzyme on the untreated surface. A thermophilic β-glucosidase immobilized with our PIII-treatment method shows an average glucose production rate more than twenty-fold higher, over five uses, compared to the commercially-available β-glucosidase immobilized by physisorption after purification.

Graphical abstract: Linker-free covalent thermophilic β-glucosidase functionalized polymeric surfaces

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Publication details

The article was received on 18 Jul 2011, accepted on 08 Sep 2011 and first published on 28 Sep 2011


Article type: Paper
DOI: 10.1039/C1JM13376D
Citation: J. Mater. Chem., 2011,21, 17832-17841
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    Linker-free covalent thermophilic β-glucosidase functionalized polymeric surfaces

    S. L. Hirsh, N. J. Nosworthy, A. Kondyurin, C. G. dos Remedios, D. R. McKenzie and M. M. M. Bilek, J. Mater. Chem., 2011, 21, 17832
    DOI: 10.1039/C1JM13376D

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