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Issue 28, 2011
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Photocontrollable DNA hybridization on reversibly photoresponsive surfaces

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Abstract

Herein we have developed a new DNA sensor which displays the possibility of photocontrollable DNA hybridization by changing the orientation of azobenzene layers on the silicon wafer surface. Basically, the trans-form layers present a coordinating surface, the “on” state that can be switched “off” in the cis-form. Water contact angles of the prepared substrates have been measured after UV and visible light irradiation to understand the switchable properties of each form of the surface. The photocontrollable DNA chip was prepared using Cy3 labeled amino terminated single stranded probe-DNA (Cy3–ssDNA–NH2) molecules which were immobilized onto a COOH-terminated photoswitchable surface. The optimum hybridization conditions were performed with Cy5 labeled complementary-DNA (Cy5–ssDNA) using fluorescence microscopy. Furthermore, the photocontrolling of DNA hybridization onto prepared surfaces was verified by confocal microscopy before and after light irradiation. The percentage hybridization ratios from confocal microscopy for on and off positions of the DNA sensor were calculated to be 61% and 5%, respectively. These results show that the prepared surfaces can be reversibly photoswitched between two states efficiently. As a result we believe that the results demonstrate the great potential to control DNA hybridization within and on the surfaces of molecular constructs using light.

Graphical abstract: Photocontrollable DNA hybridization on reversibly photoresponsive surfaces

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Publication details

The article was received on 20 Apr 2011, accepted on 03 May 2011 and first published on 09 Jun 2011


Article type: Paper
DOI: 10.1039/C1JM11721A
Citation: J. Mater. Chem., 2011,21, 10415-10420
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    Photocontrollable DNA hybridization on reversibly photoresponsive surfaces

    G. B. Demirel, N. Dilsiz, M. A. Ergün, M. Çakmak and T. Çaykara, J. Mater. Chem., 2011, 21, 10415
    DOI: 10.1039/C1JM11721A

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