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Issue 38, 2011
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Modulating cell-uptake behavior of Au-based nanomaterials via quantitative biomolecule modification

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Abstract

The pre-modification method provides a rapid and controllable amount of biomolecule conjugated to nanomaterials via quantitative creation of “activated sites” on biomolecules, modulating specific targeting rate toward cancer cells. In our studies, activating reagent, 2-iminothiolane, is introduced into the biomolecule structure as activated site for linking to Au nanomaterial. To fabricate Au-based magnetic nanoparticles, the amounts of Au nanoparticles are controllably conjugated to chitosan–Fe3O4 nanoparticles by increasing activated sites on chitosan, adjusted by 2-iminothiolane concentration. Further, to develop the biorecognition of Au–Fe3O4 nanoparticles toward target cells, pre-modified transferrin (Tf) is allowed to facilitate the conjugation with Au–Fe3O4 nanoparticles, and validations of bioactivity and specificity are examined in vitro using J5 cancer cells as well. The cell uptake analysis indicates that the high-degree Tf modified Au–Fe3O4 nanoparticles have rapid-targeting ability and easy internalization toward cancer cell, compared to low-degree ones. It implies that the efficiency of cell targeting can be improved or lowered by modulating Tf modification. The potential control of applications can be achieved by modulating Tf modification, such as rapid-targeting rate for signal enhancement, the long-term or rapid drug-treatment in circulation, etc. This concept of modulating by Tf modification can be extensively utilized in future designs of various biomolecule-conjugated Au-based nanomaterials.

Graphical abstract: Modulating cell-uptake behavior of Au-based nanomaterials via quantitative biomolecule modification

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Publication details

The article was received on 01 Apr 2011, accepted on 14 Jul 2011 and first published on 18 Aug 2011


Article type: Paper
DOI: 10.1039/C1JM11365H
Citation: J. Mater. Chem., 2011,21, 14821-14829
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    Modulating cell-uptake behavior of Au-based nanomaterials via quantitative biomolecule modification

    J. Huang, T. Lai, L. Cheng, R. Liu, C. H. Lee, M. Hsiao, C. Chen, L. Her and D. P. Tsai, J. Mater. Chem., 2011, 21, 14821
    DOI: 10.1039/C1JM11365H

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