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Issue 21, 2011
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Polyethylenimine-functionalized graphene oxide as an efficient gene delivery vector

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Abstract

A novel gene delivery system based on graphene oxide chemically-functionalized with branched polyethylenimine (PEI-GO) is reported. The PEI-GO conjugate was formed by the covalent linking of PEI and GO via an amide bond by widely used EDC chemistry. Thus-prepared PEI-GO exhibits an excellent ability to condense DNA at a low mass ratio with a positive potential of 49 mV. A WST assay reveals that PEI-GO is significantly less cytotoxic than PEI 25 kDa. Finally, the transfection efficiency of PEI-GO was evaluated. It is demonstrated that the luciferase expression of PEI-GO is comparable or even higher than that of the PEI 25 kDa at optimal mass ratio. Moreover, intracellular tracking of Cy3-labelled pGL-3 indicates that PEI-GO could effectively deliver plasmid DNA into cells and be localized in the nucleus. These findings suggest that PEI-GO is a promising candidate for efficient gene delivery.

Graphical abstract: Polyethylenimine-functionalized graphene oxide as an efficient gene delivery vector

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Publication details

The article was received on 22 Jan 2011, accepted on 22 Mar 2011 and first published on 13 Apr 2011


Article type: Paper
DOI: 10.1039/C1JM10341E
Citation: J. Mater. Chem., 2011,21, 7736-7741
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    Polyethylenimine-functionalized graphene oxide as an efficient gene delivery vector

    B. Chen, M. Liu, L. Zhang, J. Huang, J. Yao and Z. Zhang, J. Mater. Chem., 2011, 21, 7736
    DOI: 10.1039/C1JM10341E

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