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Issue 18, 2011
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Easy access to efficient magnetically recyclable separation of histidine-tagged proteins using superparamagnetic nickel ferrite nanoparticle clusters

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Abstract

The simple and efficient separation of histidine-tagged (His-tagged) proteins using nickel ferrite (NiFe2O4) nanoparticle clusters (NPCs) is described in this article. While nanostructured materials containing Ni2+ ions for efficient separation were generally synthesized via complex synthetic procedures, the NiFe2O4 nanoparticle clusters used in this work were simply synthesized by a one-pot hydrothermal polyol process. Their large surface area (105.0 m2 g−1) and pore volume (0.32 cm3 g−1) provide sufficient sites for the separation of His-tagged proteins. The high magnetic saturation value (41.3 emu g−1) and superparamagnetic property of these nanoparticle clusters lead to a more efficient magnetically recyclable separation of His-tagged proteins. We confirmed that the binding capacity and selective separation ability seen in the first separation were strongly maintained for up to 7 cycles.

Graphical abstract: Easy access to efficient magnetically recyclable separation of histidine-tagged proteins using superparamagnetic nickel ferrite nanoparticle clusters

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Publication details

The article was received on 25 Nov 2010, accepted on 07 Mar 2011 and first published on 30 Mar 2011


Article type: Paper
DOI: 10.1039/C0JM04089D
Citation: J. Mater. Chem., 2011,21, 6713-6717
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    Easy access to efficient magnetically recyclable separation of histidine-tagged proteins using superparamagnetic nickel ferrite nanoparticle clusters

    J. Chun, S. W. Seo, G. Y. Jung and J. Lee, J. Mater. Chem., 2011, 21, 6713
    DOI: 10.1039/C0JM04089D

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