Jump to main content
Jump to site search

Issue 11, 2011
Previous Article Next Article

Iodine as an elemental marker for imaging of single cells and tissue sections by laser ablation inductively coupled plasma mass spectrometry

Author affiliations

Abstract

A new laser ablation (LA)-ICP-MS method for single cell and cell nucleus imaging was developed. Therein, iodine was employed as an elemental dye for fibroblast cells and for thin tissue sections. At an incubation time of 60 s, iodine is located mainly within the cell nuclei. This effect was illustrated in fibroblast cells, and iodine signal within the cell nucleus was as high as 5 × 104 cps at 4 μm laser spot size. The surrounding cytoplasm was iodinated as well, but to a lesser extent. The spatial resolution attained was sufficient to detect even smaller cell nuclei within a liver biopsy tissue. Furthermore, iodine was successfully employed for biomolecule labeling and we demonstrated that iodine signal increased with increasing thickness of a palatine tonsil tissue. Thus, the use of iodine as an internal standard to correct for tissue inhomogeneities in LA-ICP-MS was investigated for the simultaneous detection of two tumor markers (Her 2 and CK 7) in breast cancer tissue. Additionally, lanthanide background resulting from glass ablation can be corrected for by Eu standardization.

Graphical abstract: Iodine as an elemental marker for imaging of single cells and tissue sections by laser ablation inductively coupled plasma mass spectrometry

Back to tab navigation

Publication details

The article was received on 01 Aug 2011, accepted on 07 Sep 2011 and first published on 22 Sep 2011


Article type: Paper
DOI: 10.1039/C1JA10227C
Citation: J. Anal. At. Spectrom., 2011,26, 2160-2165
  •   Request permissions

    Iodine as an elemental marker for imaging of single cells and tissue sections by laser ablation inductively coupled plasma mass spectrometry

    C. Giesen, L. Waentig, T. Mairinger, D. Drescher, J. Kneipp, P. H. Roos, U. Panne and N. Jakubowski, J. Anal. At. Spectrom., 2011, 26, 2160
    DOI: 10.1039/C1JA10227C

Search articles by author

Spotlight

Advertisements