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Issue 1, 2011
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Full quantification of selenium species by RP and AF-ICP-qMS with on-line isotope dilution in serum samples from mercury-exposed people supplemented with selenium-enriched yeast

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Abstract

Accurate determination of selenium (Se) species in biological samples is a critical issue because Se commonly occurs at low levels and in diverse species. The method for the full quantification of Se species in serum samples was proposed through combined ion-pair reverse-phase (RP) chromatography and affinity chromatography (AF) hyphenated to inductively coupled plasma-(quadrupole) mass spectrometry (ICP-qMS) with post-column isotope dilution analysis (IDA) and a collision cell technique (CCT). Different Se species like inorganic Se (Se4+ and Se6+), selenocystine (SeCys), selenomethionine (SeMet), selenoprotein P (SelP), selenoalbumin (SeAlb) and glutathione peroxidase (GPx) can be separated and quantified. The proposed methodology was used to qualitatively and quantitatively study the dynamic distribution of Se species in human serum samples from the Hg-contaminated area after supplementation with 100 μg of Se daily as Se-enriched yeast for 180 days. SelP takes up almost half and even more of the total Se and increases with the Se administration. The repeatability in terms of relative standard deviation (R.S.D. %, n = 10) is 6% for GPx and SelP and 5% for SeAlb. The detection limits are 0.1 μg Se L−1 for GPx and other non-retained Se compounds, 1.0 μg Se L−1 for SelP and 1.2 μg Se L−1 for SeAlb, 1.3 μg Se L−1 for inorganic Se; 1.2 μg Se L−1 for SeCys; 1.1 μg Se L−1 for SeMet, respectively.

Graphical abstract: Full quantification of selenium species by RP and AF-ICP-qMS with on-line isotope dilution in serum samples from mercury-exposed people supplemented with selenium-enriched yeast

  • This article is part of the themed collection: Speciation
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Publication details

The article was received on 20 Aug 2010, accepted on 05 Nov 2010 and first published on 25 Nov 2010


Article type: Technical Note
DOI: 10.1039/C0JA00129E
Citation: J. Anal. At. Spectrom., 2011,26, 224-229
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    Full quantification of selenium species by RP and AF-ICP-qMS with on-line isotope dilution in serum samples from mercury-exposed people supplemented with selenium-enriched yeast

    Y. Li, L. Hu, B. Li, X. Huang, E. H. Larsen, Y. Gao, Z. Chai and C. Chen, J. Anal. At. Spectrom., 2011, 26, 224
    DOI: 10.1039/C0JA00129E

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