Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 10, 2011
Previous Article Next Article

Microtopographical assembly of cardiomyocytes

Author affiliations

Abstract

One of the central challenges in cardiac tissue engineering is the control of the assembly and organization of functional cardiac tissue. Maintenance of a three-dimensional tissue architecture is key to myocardial function in vivo, and a variety of studies hint that provision of topological cues within scaffolds can facilitate the engineering of functional myocardial tissue by promoting this architecture. To explore this possibility in an isolated and well-defined fashion, we have designed scaffolds of polydimethylsiloxane (PDMS) with microtopographic pillars (“micropegs”) to provide cells with defined structures with which to interact in three dimensions. We show that these surfaces permit HL-1 cardiomyocytes to grow, form myofibrillar structures and cell–cell adhesions, and beat spontaneously. Additionally, the cells and their nuclei interact with the full length of the micropegs, indicating that the micropegs promote a three-dimensional cytoarchitecture in the context of a two-dimensional scaffold. We also show that the number of cells interacting with a micropeg can be controlled by manipulating incubation time, micropeg spatial arrangement, or micropeg diameter. Western blots reveal that the expression of the junctional markers N-cadherin and connexin 43 is upregulated in the presence of specific arrangements of micropegs, suggesting that micropegs can enhance cardiomyocyte function. Together, these data show that microtopography can be used to provide three-dimensional adhesion and control the assembly of functional cardiac tissue on a two-dimensional surface.

Graphical abstract: Microtopographical assembly of cardiomyocytes

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 12 Mar 2011, accepted on 04 Aug 2011 and first published on 24 Aug 2011


Article type: Paper
DOI: 10.1039/C1IB00024A
Citation: Integr. Biol., 2011,3, 1011-1019
  •   Request permissions

    Microtopographical assembly of cardiomyocytes

    A. A. Patel, T. A. Desai and S. Kumar, Integr. Biol., 2011, 3, 1011
    DOI: 10.1039/C1IB00024A

Search articles by author