Multi-modal molecular imaging approaches to detect primary cells in preclinical models

Kevin Dhaliwal; Lois Alexander; Geraldine Escher; Asier Unciti-Broceta; Maurits Jansen; Neil Mcdonald; Juan M. Cardenas-Maestre; Rosario Sanchez-Martin; John Simpson; Chris Haslett,; Mark Bradley

doi:10.1039/C005410K

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Faraday Discussions

Issue 0, 2011

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Multi-modal molecular imaging approaches to detect primary cells in preclinical models

Kevin Dhaliwal , Lois Alexander , Geraldine Escher , Asier Unciti-Broceta , Maurits Jansen , Neil Mcdonald , Juan M. Cardenas-Maestre , Rosario Sanchez-Martin , John Simpson , Chris Haslett, and Mark Bradley

Faraday Discuss., 2011,149, 107-114

DOI: 10.1039/C005410K
Received 01 Jun 2010, Accepted 05 Jul 2010
First published on the web 15 Oct 2010
This article is part of the collection: Analysis for Healthcare Diagnostics and Theranostics

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The need to understand cellular trafficking in vivo in situ requires the development and application of novel methodologies for cellular labeling and cell tracking. Here we applied new technologies associated with advances in molecular imaging to demonstrate the feasibility of labeling primary immune cells. We demonstrate the utility of fluorescently tagged polystyrene microspheres, MRI susceptible emulsions and cell entry peptoids. The adaptation of these labeling agents will permit cell specific delivery, diagnostic sensing and the delivery of therapeutic agents to sites of inflammation and infection.

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